Tick protocol - Revision history

imported>Weigang at 23:31, 15 July 2021

2021-07-15T23:31:07Z

← Older revision		Revision as of 23:31, 15 July 2021
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	=Nymph tick collection=		=Nymph tick collection=
	* Questing seasson: June 10 - July 10 in Northeast US		* Questing seasson: June 10 - July 10 in Northeast US
			* [https://www.bayarealyme.org/wp-content/uploads/2014/08/tick_size_Western_Eastern_color-REV.jpg Tick sizes]
	* Habitat:		* Habitat:
	** oak and pine forest (not grassland)		** oak and pine forest (not grassland)

imported>Weigang at 21:31, 14 July 2021

2021-07-14T21:31:37Z

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 [[File:Nymph-3.jpg|thumbnail]]
 =Nymph tick maintenance & transportation=
 * Date: July 12, 2021

imported>Weigang at 21:26, 14 July 2021

2021-07-14T21:26:58Z

← Older revision		Revision as of 21:26, 14 July 2021
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			[[File:Nymph-3.jpg\|thumbnail]]
	=Nymph tick maintenance & transportation=		=Nymph tick maintenance & transportation=
	~~[[File:Nymph-3.jpg\|thumbnail]]~~
	* Date: July 12, 2021		* Date: July 12, 2021
	* Draft: Brian Sulkow		* Draft: Brian Sulkow

imported>Weigang: /* Nymph tick maintenance & transportation */

2021-07-14T21:26:08Z

Nymph tick maintenance & transportation

← Older revision		Revision as of 21:26, 14 July 2021
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	=Nymph tick maintenance & transportation=		=Nymph tick maintenance & transportation=
			[[File:Nymph-3.jpg\|thumbnail]]
	* Date: July 12, 2021		* Date: July 12, 2021
	* Draft: Brian Sulkow		* Draft: Brian Sulkow

imported>Weigang: /* Protocol */

2021-07-14T20:59:30Z

Protocol

imported>Weigang: /* Protocol */

2021-07-14T20:59:06Z

Protocol

← Older revision		Revision as of 20:59, 14 July 2021
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	** Sterile (enough) paper towels- open a new roll of paper towels.		** Sterile (enough) paper towels- open a new roll of paper towels.
	==Protocol==		==Protocol==
	~~Part 1:~~ Prepare tick vials with Plaster of Paris		# Prepare tick vials with Plaster of Paris
	1) Use directions on the Plaster of Paris (PP) package to prepare an appropriate amount.		## Use directions on the Plaster of Paris (PP) package to prepare an appropriate amount.
	2) Transfer wet PP to a vial: fill 1/8 to ¼ of an empty vial, from the base up, with wet PP. Note: Avoid getting PP on the walls of the vial above the mark you filled it to.		## Transfer wet PP to a vial: fill 1/8 to ¼ of an empty vial, from the base up, with wet PP. Note: Avoid getting PP on the walls of the vial above the mark you filled it to.
	3) Allow PP to dry: drying time for PP is 30 minutes to 2 hours. Ticks are put into the vial only after PP dries. Note-drying time differs from curing time.		## Allow PP to dry: drying time for PP is 30 minutes to 2 hours. Ticks are put into the vial only after PP dries. Note-drying time differs from curing time.
	4) Drops of Water: Using a dropper prior to transferring ticks to a vial, put <font color="red">2-3 drops</font> (pipette 1.5 tips) of sterile water on the PP in the vial.		## Drops of Water: Using a dropper prior to transferring ticks to a vial, put <font color="red">2-3 drops</font> (pipette 1.5 tips) of sterile water on the PP in the vial.
	~~Part 2:~~ Transfers ticks and cover the vial
	1) Using tweezers, transfer up to 30 ticks into a vial prepared with PP.		# Transfers ticks and cover the vial
	2) Cover vial with mesh gauze: When ticks are transferred, cover the vial nylon mesh. Fasten mesh over the vial with a rubber band.		## Using tweezers, transfer up to 30 ticks into a vial prepared with PP.
	3) Cuts in the mesh: Make cuts in the cap to allow air flow		## Cover vial with mesh gauze: When ticks are transferred, cover the vial nylon mesh. Fasten mesh over the vial with a rubber band.
	~~Part 3:~~ Transfer vials with ticks to a zip-lock bag		## Cuts in the mesh: Make cuts in the cap to allow air flow
	1) Avoid suffocation: Vials should be placed upright into the Ziploc bag. To reduce the chance of suffocating the ticks, place vials in such a way that the mesh on top is not covered by the plastic walls of the zip-lock bag.
	2) Size of towel: From a larger paper towel, cut out a 2”x2” square. Fold the square a few times.		# Transfer vials with ticks to a zip-lock bag
	3) Water on the towel: Add <font color="red">0.5 ml</font> of sterile water to the folded square.		## Avoid suffocation: Vials should be placed upright into the Ziploc bag. To reduce the chance of suffocating the ticks, place vials in such a way that the mesh on top is not covered by the plastic walls of the zip-lock bag.
	4) Towel placement: Place the towel to the Ziploc bags with the ticks. To reduce the chances of suffocating ticks, do not place the towel on mesh covers for vials, rather place the towel on side of the bag or the bottom of the bag.		## Size of towel: From a larger paper towel, cut out a 2”x2” square. Fold the square a few times.
	~~Part 4:~~ Packaging		## Water on the towel: Add <font color="red">0.5 ml</font> of sterile water to the folded square.
	1) Take air out of the bag: Take most of the air out of the bag and close the Ziploc bag. To avoid suffocation, during this process do not let the walls of the bag cover the mesh tops of the vials.		## Towel placement: Place the towel to the Ziploc bags with the ticks. To reduce the chances of suffocating ticks, do not place the towel on mesh covers for vials, rather place the towel on side of the bag or the bottom of the bag.
	2) Before sending: Keep the closed Ziploc bag at room temperature, in a place with natural light, but out of direct sunlight.
	3) Boxing: In the late afternoon, when you are ready to take the Ziplock bags to FedEx, put the prepared Ziplock bags in a box. To avoid breaking vials when shipping, surround the Ziplock bag with packaging materials.		# Packaging
	4) Over Night Shipping Instruction: To lessen the likelihood of ticks being subject to extreme heat during shipping, bring boxed ticks to a Fed-Ex shipping center in the late afternoon. Arrange for next day delivery, before noon. Use the <font color="red">“First O/N”</font> option.		## Take air out of the bag: Take most of the air out of the bag and close the Ziploc bag. To avoid suffocation, during this process do not let the walls of the bag cover the mesh tops of the vials.
			## Before sending: Keep the closed Ziploc bag at room temperature, in a place with natural light, but out of direct sunlight.
			## Boxing: In the late afternoon, when you are ready to take the Ziplock bags to FedEx, put the prepared Ziplock bags in a box. To avoid breaking vials when shipping, surround the Ziplock bag with packaging materials.
			## Over Night Shipping Instruction: To lessen the likelihood of ticks being subject to extreme heat during shipping, bring boxed ticks to a Fed-Ex shipping center in the late afternoon. Arrange for next day delivery, before noon. Use the <font color="red">“First O/N”</font> option.

imported>Weigang: /* Materials */

2021-07-14T19:48:14Z

Materials

← Older revision		Revision as of 19:48, 14 July 2021
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	* Consultation: Jose Filipe G T Cabral De Azevedo [UTHSC]		* Consultation: Jose Filipe G T Cabral De Azevedo [UTHSC]
	==Materials==		==Materials==
	** Flask made of glass or non-porous plastic		* Flask made of glass or non-porous plastic
	** Plaster of Paris		* Plaster of Paris
	** Dropper/pipette		* Dropper/pipette
	** Nylon mesh with holes small enough so that ticks don’t escape		* Nylon mesh with holes small enough so that ticks don’t escape
	** Rubber bands		* Rubber bands
	** Tweezers		* Tweezers
	** Sterile water		* Sterile water
	** Clean paper towels		* Clean paper towels
	** Ziploc bags		* Ziploc bags
	** Packaging material & box for shipping		* Packaging material & box for shipping

	==Storage==		==Storage==
	* When at your home, ticks are kept at room temperature, in a room with natural light, but out of direct sunlight.		* When at your home, ticks are kept at room temperature, in a room with natural light, but out of direct sunlight.

imported>Weigang: Created page with "=Nymph tick maintenance & transportation= * Date: July 12, 2021 * Draft: Brian Sulkow * Consultation: Jose Filipe G T Cabral De Azevedo [UTHSC] ==Materials== ** Flask made of..."

2021-07-14T19:47:46Z

Created page with "=Nymph tick maintenance & transportation= * Date: July 12, 2021 * Draft: Brian Sulkow * Consultation: Jose Filipe G T Cabral De Azevedo [UTHSC] ==Materials== ** Flask made of..."

New page

=Nymph tick maintenance & transportation=
* Date: July 12, 2021
* Draft: Brian Sulkow
* Consultation: Jose Filipe G T Cabral De Azevedo [UTHSC]
==Materials==
** Flask made of glass or non-porous plastic
** Plaster of Paris
** Dropper/pipette
** Nylon mesh with holes small enough so that ticks don’t escape
** Rubber bands
** Tweezers
** Sterile water
** Clean paper towels
** Ziploc bags
** Packaging material & box for shipping
==Storage==
* When at your home, ticks are kept at room temperature, in a room with natural light, but out of direct sunlight.
* Avoid the use of ice packs, which cause condensation and flooding of the vials
* Avoid bacterial growth in tick containers:
** Wear Gloves or Wash hands thoroughly.
** Sterile water- use sterile water (boil water for 5 mins, let cool, store in a sterile jar). Use bottled water.
** Sterile (enough) paper towels- open a new roll of paper towels.
==Protocol==
Part 1: Prepare tick vials with Plaster of Paris
1) Use directions on the Plaster of Paris (PP) package to prepare an appropriate amount.
2) Transfer wet PP to a vial: fill 1/8 to ¼ of an empty vial, from the base up, with wet PP. Note: Avoid getting PP on the walls of the vial above the mark you filled it to.
3) Allow PP to dry: drying time for PP is 30 minutes to 2 hours. Ticks are put into the vial only after PP dries. Note-drying time differs from curing time.
4) Drops of Water: Using a dropper prior to transferring ticks to a vial, put 2-3 drops (pipette 1.5 tips) of sterile water on the PP in the vial.
Part 2: Transfers ticks and cover the vial
1) Using tweezers, transfer up to 30 ticks into a vial prepared with PP.
2) Cover vial with mesh gauze: When ticks are transferred, cover the vial nylon mesh. Fasten mesh over the vial with a rubber band.
3) Cuts in the mesh: Make cuts in the cap to allow air flow
Part 3: Transfer vials with ticks to a zip-lock bag
1) Avoid suffocation: Vials should be placed upright into the Ziploc bag. To reduce the chance of suffocating the ticks, place vials in such a way that the mesh on top is not covered by the plastic walls of the zip-lock bag.
2) Size of towel: From a larger paper towel, cut out a 2”x2” square. Fold the square a few times.
3) Water on the towel: Add 0.5 ml of sterile water to the folded square.
4) Towel placement: Place the towel to the Ziploc bags with the ticks. To reduce the chances of suffocating ticks, do not place the towel on mesh covers for vials, rather place the towel on side of the bag or the bottom of the bag.
Part 4: Packaging
1) Take air out of the bag: Take most of the air out of the bag and close the Ziploc bag. To avoid suffocation, during this process do not let the walls of the bag cover the mesh tops of the vials.
2) Before sending: Keep the closed Ziploc bag at room temperature, in a place with natural light, but out of direct sunlight.
3) Boxing: In the late afternoon, when you are ready to take the Ziplock bags to FedEx, put the prepared Ziplock bags in a box. To avoid breaking vials when shipping, surround the Ziplock bag with packaging materials.
4) Over Night Shipping Instruction: To lessen the likelihood of ticks being subject to extreme heat during shipping, bring boxed ticks to a Fed-Ex shipping center in the late afternoon. Arrange for next day delivery, before noon. Use the “First O/N” option.

← Older revision		Revision as of 20:59, 14 July 2021
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	## Allow PP to dry: drying time for PP is 30 minutes to 2 hours. Ticks are put into the vial only after PP dries. Note-drying time differs from curing time.		## Allow PP to dry: drying time for PP is 30 minutes to 2 hours. Ticks are put into the vial only after PP dries. Note-drying time differs from curing time.
	## Drops of Water: Using a dropper prior to transferring ticks to a vial, put <font color="red">2-3 drops</font> (pipette 1.5 tips) of sterile water on the PP in the vial.		## Drops of Water: Using a dropper prior to transferring ticks to a vial, put <font color="red">2-3 drops</font> (pipette 1.5 tips) of sterile water on the PP in the vial.

	# Transfers ticks and cover the vial		# Transfers ticks and cover the vial
	## Using tweezers, transfer up to 30 ticks into a vial prepared with PP.		## Using tweezers, transfer up to 30 ticks into a vial prepared with PP.
	## Cover vial with mesh gauze: When ticks are transferred, cover the vial nylon mesh. Fasten mesh over the vial with a rubber band.		## Cover vial with mesh gauze: When ticks are transferred, cover the vial nylon mesh. Fasten mesh over the vial with a rubber band.
	## Cuts in the mesh: Make cuts in the cap to allow air flow		## Cuts in the mesh: Make cuts in the cap to allow air flow

	# Transfer vials with ticks to a zip-lock bag		# Transfer vials with ticks to a zip-lock bag
	## Avoid suffocation: Vials should be placed upright into the Ziploc bag. To reduce the chance of suffocating the ticks, place vials in such a way that the mesh on top is not covered by the plastic walls of the zip-lock bag.		## Avoid suffocation: Vials should be placed upright into the Ziploc bag. To reduce the chance of suffocating the ticks, place vials in such a way that the mesh on top is not covered by the plastic walls of the zip-lock bag.
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	## Water on the towel: Add <font color="red">0.5 ml</font> of sterile water to the folded square.		## Water on the towel: Add <font color="red">0.5 ml</font> of sterile water to the folded square.
	## Towel placement: Place the towel to the Ziploc bags with the ticks. To reduce the chances of suffocating ticks, do not place the towel on mesh covers for vials, rather place the towel on side of the bag or the bottom of the bag.		## Towel placement: Place the towel to the Ziploc bags with the ticks. To reduce the chances of suffocating ticks, do not place the towel on mesh covers for vials, rather place the towel on side of the bag or the bottom of the bag.

	# Packaging		# Packaging
	## Take air out of the bag: Take most of the air out of the bag and close the Ziploc bag. To avoid suffocation, during this process do not let the walls of the bag cover the mesh tops of the vials.		## Take air out of the bag: Take most of the air out of the bag and close the Ziploc bag. To avoid suffocation, during this process do not let the walls of the bag cover the mesh tops of the vials.