Year 2019: Difference between revisions
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imported>Lab |
imported>Lab |
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| Line 3: | Line 3: | ||
* Main computational steps: | * Main computational steps: | ||
# Quantile normalization between all replicates (with log2 transformatuon) | # Quantile normalization between all replicates (with log2 transformatuon) | ||
# | # Linear model among the 3 groups | ||
# Select top significant genes | # Select top significant genes | ||
# Map gene names | # Map gene names | ||
# Cluster and show by interactive heatmaps in R | # Cluster and show by interactive heatmaps in R | ||
## | ## [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html Heatmap 1. Scaled by row] | ||
## [http://diverge.hunter.cuny.edu/~weigang/heatmap_fc_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-3). Values are Fold Change over mean Wild Tyoe] | ## [http://diverge.hunter.cuny.edu/~weigang/heatmap_fc_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-3). Values are Fold Change over mean Wild Tyoe] | ||
Revision as of 21:19, 7 February 2019
TD Project
- Credit: Christopher Panlasigui
- Main computational steps:
- Quantile normalization between all replicates (with log2 transformatuon)
- Linear model among the 3 groups
- Select top significant genes
- Map gene names
- Cluster and show by interactive heatmaps in R