Biol425 2011: Difference between revisions

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Hunter College of the City University of New York
Hunter College of the City University of New York
<span style="font-weight:bold;font-size:large;">The best way to track changes made to this page</span> is to click on the History tab above, and add the RSS feed that shows up there in your browser. If you use Chrome, please see [[#Useful Links]] below for two very handy extensions if you don't have them, or other RSS extensions, already.


==Course information==
==Course information==
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*Classroom Q & A (5%):  Read the chapters before lecture.
*Classroom Q & A (5%):  Read the chapters before lecture.
*Attendance (5%): 1-2 absences = -2.5%. More than 2 = -5%.
*Attendance (5%): 1-2 absences = -2.5%. More than 2 = -5%.
===Programming Assignment Expectations===
All code must begin with the lines in the Perl slides, without exception. For each assignment, unless otherwise stated, I would like the '''full text''' of the source code. Since you cannot print using the text editor in the lab (even if you are connected from home), you must copy and paste the code into a word processor or a local text editor. If you are using a word processor, '''change the font to a fixed-width/monospace font'''. On Windows, this is usually '''Courier'''.
Code indentation is '''your personal taste''', so long as it is consistent and readable. Use comments whenever you think either the code is unclear, or simply as a guideline for yourself. Well-commented code improves readability, but be careful not overdo it.
Also, unless otherwise stated, both the '''input and the output of the program must be submitted as well'''. This should '''also be in fixed-width font''', and you should '''label''' it in such a way so that I know it is the program's input/output. This is so that I know that you've run the program, what data you have used, and what the program produced.
If you are working from the lab, one option is to email the code to yourself, change the font, and then print it somewhere else as there is no printer in the lab.


==Course Schedule (All Saturdays)==
==Course Schedule (All Saturdays)==
Line 61: Line 73:


'''"Lecture slides" links will be available either during or before each lecture, in PDF.'''
'''"Lecture slides" links will be available either during or before each lecture, in PDF.'''
'''Homework assignments are due the week *after* the date under which they appear.''' ie, an assignment posted under Jan 29 is due the following lecture, on Feb 5.


===January 29===
===January 29===
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*'''UNIX Tutorial:''' Please check the new '''[[#Useful Links]]''' section below
*'''UNIX Tutorial:''' Please check the new '''[[#Useful Links]]''' section below
*'''How to connect remotely:''' ([http://qiu.bioweb.hunter.cuny.edu/index.php?option=com_content&view=article&id=110 Windows]) ([http://qiu.bioweb.hunter.cuny.edu/index.php?option=com_content&view=article&id=111 Mac])
*'''How to connect remotely:''' ([http://qiu.bioweb.hunter.cuny.edu/index.php?option=com_content&view=article&id=110 Windows]) ([http://qiu.bioweb.hunter.cuny.edu/index.php?option=com_content&view=article&id=111 Mac])
* '''Homework:'''
* '''Homework: This homework will *not* be graded. It is for practice purposes ONLY.'''
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{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
|- style="background-color:lightsteelblue;"
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===February 5===
===February 5===
*Chapter 1. Central Dogma & Wet Lab Tools [Lecture Slides]
*'''Chapter 1.''' Central Dogma & Wet Lab Tools [[Media:Molecular_Biology_and_Genomics.pdf|Lecture Slides Ch.1-Che]]
*Homework:
*'''Beginning Perl''' ([[Media:Bio425_beginning_perl.pdf‎|Beginning Perl, Part 1 Slides]])
*'''Homework:''' (this assignment *will* be graded.)
{| class="collapsible collapsed wikitable"
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
|- style="background-color:lightsteelblue;"
! Assignment #2
! Assignment #2
|- style="background-color:powderblue;"
|- style="background-color:powderblue;"
| '''Linux and Molecular Sequences'''<br />
| '''Before you begin...'''<br />
#Stay in your home directory (without changing into the "bio425_2011" directory), count the number of ORFs in the sequence file "GBB.seq" using a single "grep" command and one option
Do this ONLY ONCE: <pre>echo "source /data/yoda/b/student.accounts/bio425_2011/bio425.profile" >> ~/.bash_profile</pre>
#Achieve the same purpose as above, by using "grep" and "wc"
Alternatively, you can open ~/.bash_profile in a text editor (ask me if don't know how) and paste the line: <pre>source /data/yoda/b/student.accounts/bio425_2011/bio425.profile</pre> at the end.
#Again, stay in your home directory, filter the DNA sequences in the file "/data/yoda/b/student.accounts/bio425_2011/data/TenSeq.nuc" into upper cases
|-style="background-color:powderblue;"
#Filter out the "a"s in the above sequence
| '''Beginning Perl'''<br />
#Using "sed", filter out the string "plasmid" in the sequence names from the file "/data/yoda/b/student.accounts/bio425_2011/data/GBB.1con"
For the homework, read up to page 221 in Appendix 1. For February 26, read all of Appendix 1.
#Using "cut", extract the third field delimited by "_"
 
#Compose regular expressions matching the Thr, Ser, and Glu codons, respectively
There are '''two choices''' for the homework. The first is recommended for novices. The second is for those who are either comfortable with Perl, or feel the need for a challenge this early. Only complete ONE of these assignments, as I will only accept one. Please follow the guidelines listed [[#Programming Assignment Expectations|above]].
#Write a BASH loop to count the number of sequences in every *.pep file in the directory "/data/yoda/b/student.accounts/bio425_2011/data/"
 
# Copy the code from page 221 in a new file. (Remember to put the code from the slides in the beginning of the file and to declare all variables on first use!) You must alter the code so that the resulting program accomplishes the following four tasks:
##Instead of taking the average of 10 numbers, ask the user how many numbers to average and use that number instead. (Hint: see how the code asks for each number). This must be stored in a new variable.
##If the number the user gave was 0 or negative, print a message telling the user so, and exit immediately. You can exit using <pre>exit;</pre>
##The code always prints 'Enter another number:'. Change it so that on the '''first time only''' it instead prints 'Enter a number:'.
##Just before printing the average, print a message saying 'The numbers to average are: '. Then print out out all the numbers the user entered.
#More advanced programmers can try this assignment (you may wish to read all of Appendix 1 now): create a script which can take as input one or more DNA sequences from a file and translate directly to the correct amino acid sequence (single-letter format). You may implement this program in Perl however you wish, with as much complexity as you wish, as long as it meets the guidelines above and satisfies the following four criteria:
##The format of the input file it reads must be: one DNA sequence per line, so that each DNA sequence is separated by a new line character. '''Also assume you are given the coding strand.'''
##The name of the input file cannot be hard coded. You may either ask the user for the file location/name or take it as a command line argument.
##It must tolerate all upper-case, lower-case or mixed-case sequences in the input
##For every input DNA sequence, output the DNA sequence, the equivalent RNA, and the peptide sequence. The output '''must''' be informative, ie:
##:Input: atgcgtcgataa
##:Output: augcgucgauaa
##:Peptide: MRR*
#:Additionally, the program cannot use any outside dependencies/modules such as BioPerl (supposing you know how to use it.) Also note that STOP codons are denoted by a '<nowiki>*</nowiki>'
|-style="background-color:powderblue;"
|-style="background-color:powderblue;"
| '''Problems'''<br />
| '''Problems'''<br />
(pg.31-32): 1.2, 1.3, 1.5, 1.7, 1.9, 1.10, 1.11
(pg.31-32): 1.2, 1.3, 1.5,1.9, 1.10, 1.11
|}
|}


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'''Yozen will not be lecturing'''
'''Yozen will not be lecturing'''


*Chapter 6. Gene and Genome Structures [Lecture Slides]
*Chapter 6. Gene and Genome Structures [Lecture Slides [[Media:Chapter_6.pdf|Lecture Slides Ch.6-Che]]
*'''Tutorial:''' ORF Prediction using GLIMMER
*'''Tutorial:''' ORF Prediction using GLIMMER
<!--*Assignment #3. '''(To be posted)'''
* '''Homework:''' This homework will be graded.
Gene Identification, read Appendix 1-->
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #3
|- style="background-color:powderblue;"
| '''Bacterial gene identification using Glimmer'''<br />
Remember to first log in to mysql by doing: <pre>ssh mysql</pre>
#Copy the Lyme disease bacterium lp17 plasmid file "/data/yoda/b/student.accounts/bio425_2011/data/lp17.fas" into your home directory.
#Run long-orf, extract, build-icm, and glimmer3.
#Show your commands and "cat" the final output.
#Describe key elements of a prokaryotic gene in addition to the open reading frame.
#Textbook Questions (pg152-153): 6.6, 6.9, 6.15
|-style="background-color:powderblue;"
| '''Read''' All of Appendix 1.
|}


===February 26===
===February 26===
*Appendix 1. Basic PERL [Lecture Slides]
*Appendix 1. More PERL ([[Media:Bio425_more_perl.pdf|Lecture Slides]])
<!--*Assignment #4. '''(To be posted)'''
*'''Homework'''
Write a PERL script to print the reverse complementary strand of a DNA sequence (print the original, complement, and reverse complement strings; 5 pts). *Questions & Problems (pg.234): A1.2 (5 pts)-->
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #4
|-style="background-color:powderblue;"
| '''Beginning Perl'''<br />
This time, both novices and experienced programmers do the same homework, with one small difference in the use of the program.
 
Recall from the first class where I introduced the FASTA-format. In this format, sequence data is recorded as follows:
<pre>>SequenceID_info1_info2
atgcgtgatg...</pre>
 
Of course, the ID portion is itself not standardized, and the sequence can also be an amino acid sequence. For simplicity, let's assume that in the ID field, you have a "Strain" name followed by a "protein" name, separated by an underscore (_). You will write a program to read a FASTA file with the ID format described above, and a nucleotide sequence. For both novice-level and experienced level programmers, your program will:
 
# Pick out the strain name, the protein name, and the nucleotide sequence.
# Calculate he length of each sequence.
# Calculate the GC content (in percent) of each sequence.
# Calculate the percent composition of each nucleotide (base composition).
 
'''Novice-level task:'''
 
Your program will just print the above information '''for all sequences''', in a readable form. Sample output could be:
<pre>Strain: B31
Protein: ospA
Seq Length: 819
GC content: 33.58%
Base composition: A 42.98 %, T 23.44 %, C 14.77 %, G 18.80 %</pre>
If your percentages have more than 2 decimal places, '''that's OK.'''
 
'''Experienced-level task:'''
 
The only difference from novices is that your program will '''ask the user for the name of a strain and protein, separated by an underscore''' (ie, B31_opsA). Once given that input, it will print the exact same output as above, but only for the sequence described by that input. If the input doesn't exist, it will say so and exit. Your program will '''continue to ask the user for the sequence ID''' until the user types 'quit' or they give an invalid sequence ID. You can do this by using a while loop.
 
'''Notes'''
 
Calculating the GC content and the base composition is easy if you make use of the tr (transliterate) function as described at the bottom of page 232, and divide the result by the sequence length. GC content is just the sum of total G and C nucleotides, divided by the sequence length. I do want '''percents''', so remember to multiply the results by 100 and to append a '%' at the end.
 
Getting the strain name and the protein name separately can be accomplished with the split() function (check new slides or search on the internet).
 
You will test your program the with the file /data/yoda/b/student.accounts/bio425_2011/data/Borrelia_osp.dna.fasta as input. You don't have to include the file itself with your homework, but I do still want you to copy the program output and submit it with your assignment.
 
Again, the program cannot use any outside dependencies/modules such as BioPerl (supposing you know how to use it.) Besides that, you can implement it however you like. If you know about references, '''it is possible to do this assignment without using them.'''
|}


===March 5===
===March 5===
*Object-Oriented PERL & BioPerl [Lecture Slides]
 
<!--*Assignment #5. '''(To be posted)'''
*Chapter 2. Data Search and Alignments [[Media:Chapter2.pdf|Lecture Slides Ch.2-Che]]
BioPerl-->
*Object-Oriented PERL & BioPerl (Link to [http://www.bioperl.org/wiki/Main_Page Bioperl] site and [http://www.bioperl.org/wiki/HOWTOs HOWTOs])
*'''Homework:'''
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #5
|-style="background-color:powderblue;"
| '''BioPerl Assignment'''
 
For this assignment, you will use the .predict file you made with glimmer in [[#February_19 | assignment 3]].
 
If connecting from home: open gedit '''before''' logging on to mysql.
 
For BioPerl to work, you '''must''' log on to mysql.
 
'''Complete the assignment by following these steps.''' Make sure each part works '''before''' trying to solve the next part:
# Make a perl script that reads each line from the .predict file that describes a gene (skip the heading line).
# Save each line ('''hint:''' array, anyone?)
# Now, in the same script, use '''Bio::SeqIO''' to read the lp17.fas file '''and get a Bio::Seq object.'''
# Go through each line saved from the .predict file. Remember: these are predicted orfs:
## For each of these, '''extract the start and stop positions and "strand" values''' (the three values following the orf name).
## If the strand starts with a '-', it means the orf is on the reverse complement, so you need to use the Bio::Seq method "revcom".
## Now, extract the orf sequence using the start & stop values using the Bio::Seq method "subseq", paying special attention to sequences on on the '-' strand.
## Print both the DNA sequence AND the protein sequence.
 
See these sample scripts for how to use revcom and subseq:
<pre>../bio425_2011/sample-perl-scripts/revcom_translate_seq.pl
../bio425_2011/sample-perl-scripts/subseq.pl
</pre>
 
And I linked to the HOWTO above in case you forgot.
 
'''Output should be informative:'''
<pre>
ORF: orf00002
DNA: ...
Protein: ...
</pre>
|-style="background-color:powderblue;"
| '''Read'''
'''For next class, read CH 3'''
|}


===March 12===
===March 12===
*Information Theory
*Chapter 3. Molecular Evolution [[Media:CH3.pdf|Lecture Slides Ch.3-Che]]
*Tutorial: Sequence Logo
* '''Homework:''' (TBA)
<!--*Assignment #6. '''(To be posted)'''
Information Contents at Intron-Exon junctions-->


===March 19===
===March 19===
*Chapter 2. Data Search and Alignments
*REVIEW Session for MID-TERM EXAMS
*Tutorial: Pairwise Alignment using BLAST & NUCMER
*Tutorial: Multiple Alignment using CLUSTALW
<!--*Assignment #7. '''(To be posted)'''
<!--*Assignment #7. '''(To be posted)'''
Questions & Problems (pg.54-55): 2.1, 2.2, 2.3, 2.4-->
Questions & Problems (pg.54-55): 2.1, 2.2, 2.3, 2.4-->


===March 26===
===March 26===
*Chapter 3. Molecular Evolution
*MID-TERM
<!--*Assignment #8. '''(To be posted)'''
<!--*Assignment #8. '''(To be posted)'''
Questions & Problems (pg.75-76): 3.1, 3.2, 3.3 (use first ten codons), 3.4, 3.5, 3.7-->
Questions & Problems (pg.75-76): 3.1, 3.2, 3.3 (use first ten codons), 3.4, 3.5, 3.7-->


===April 2===
===April 2===
'''NO CLASSES'''
*'''Chapter 4.''' Phylogenetics I. Distance Methods  [[Media:CH4.pdf|Lecture Slides Ch.4-Che]]
*"Tree Thinking" Puzzles - ([http://diverge.hunter.cuny.edu/~weigang/lab-website/SummerWorkshop/Baum_etal05_sup_part1.pdf Download])
*'''Tutorial:''' PROTDIST and NEIGHBOR using [http://mobyle.pasteur.fr/cgi-bin/portal.py#welcome Mobyle Pasteur]
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #6
|-style="background-color:powderblue;"
| '''Chapter 4 ''' Questions & Problems (pg.95-96): 4.1, 4.3, 4.4, 4.7, 4.8
|}


===April 9===
===April 9===
*Chapter 4. Phylogenetics I. Distance Methods
*'''Chapter 5.''' Phylogenetics II. Character-Based Methods [[Media:CH4.pdf|Lecture Slides Ch.5-Che]]
*Tutorial: PROTDIST and NEIGHBOR using [http://mobyle.pasteur.fr/cgi-bin/portal.py#welcome Mobyle Pasteur]
*'''Tutorial:''' DNAML and bootstrap analysis using [http://mobyle.pasteur.fr/cgi-bin/portal.py#welcome Mobyle Pasteur]
<!--*Assignment #9. '''(To be posted)'''
<!--*Assignment #10. '''(To be posted)'''
Questions & Problems (pg.95-96): 4.1, 4.3, 4.4, 4.7, 4.8-->
Questions & Problems (pg.115-116): 5.1, 5.2, 5.3, 5.4-->


===April 16===
===April 16===
*Chapter 5. Phylogenetics II. Character-Based Methods
*'''Topic:''' Relational Database and SQL
*Tutorial: DNAML and bootstrap analysis using [http://mobyle.pasteur.fr/cgi-bin/portal.py#welcome Mobyle Pasteur]
*'''Tutorial:''' the Borrelia Genome Database
<!--*Assignment #10. '''(To be posted)'''
*'''Homework:''' SQL-embedded PERL
Questions & Problems (pg.115-116): 5.1, 5.2, 5.3, 5.4-->
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #7
|- style="background-color:powderblue;"
| '''SQL-embedded PERL'''<br />
 
Continue work on the assignment we began in class. It is reproduced below, with some added functionality.
 
Your script will:
 
# Retrieve TEN orfs from the orf table that belong to the strain Pko.
# Find and store the sequences described by those orfs and their lengths.
# Determine if the orf is on the reference or reverse complement strand, and use that information to print the correct sequence.
# Print the orf name, sequence, and the length for each orf.
# '''In addition to printing the above information to the screen,''' write out the sequence information '''(in FASTA format)''' to a file
called "Pko_orfs.fasta". The sequence ID should be of the form:
Pko_orfname
 
Note that the above will require the use of BioPerl.
 
 
For those looking for extra challenges, you can try adding the following:
 
* Ask the user for the strain and contig *names* that they want orfs from, and only retrieve those rows. This means you must find a way
of obtaining their respective IDs from just their names. Make sure the sequence IDs are informative. They should look like this:
strainname_contigname_orfname
* If asking users for input, fail if they gave a strain or contig name which does not exist in the database.
* Also if asking users for input, the output file's name should be changed to reflect the chosen strain.
* Ask the user the minimum length the orf is allowed to be, and only print orfs as long, or longer, than what the user specifies.
 
 
Sample scripts will go up slowly, over time, including example SQL statements.
|-style="background-color:powderblue;"
| '''Questions from Text''' <br /> (pg.115-116): 5.1, 5.3
|}


===April 23===
===April 23===
*Relational Database and SQL
'''NO CLASSES''' (Spring recess)
*Tutorial: the Borrelia Genome Database
<!--*Assignment #11. '''(To be posted)'''
SQL-embedded PERL-->


===April 30===
===April 30===
*Statistics
*'''Topic:''' Statistics
*Tutorial: Statistical Visualization using R
*'''In-class exercise:''' [https://docs.google.com/document/d/1wq-s8WpqyURVeGiLUxhEyBvHRDrK__Cr7XjkuLicP-c/edit?hl=en&authkey=CJ2g4qsI R basics and short demonstration of a simple boxplot]
*'''Tutorial:''' Statistical Visualization using R [[Media:R-implementations.pdf|Lecture Slides-Che]]
<!--*Assignment #12. '''(To be posted)'''
<!--*Assignment #12. '''(To be posted)'''
R Exercises-->
R Exercises-->


===May 7===
===May 7===
*Chapter 6 (Gene Expression) & Chapter 8 (Proteomics)
*'''Chapter 6''' (Gene Expression) & '''Chapter 8''' (Proteomics)
*Tutorial: Array Data Visualization and Analysis
*'''Tutorial:''' Array Data Visualization and Analysis ([[Media:Array_Data_Visualization_and_Analysis.pdf| Micro-Array Analysis Slides]])
<!--*Assignment #13. '''(To be posted)'''
*'''Homework:'''Data Analysis using R
Gene Expression Data Analysis using R-->
{| class="collapsible collapsed wikitable"
|- style="background-color:lightsteelblue;"
! Assignment #8
|-style="background-color:powderblue;"
| '''Part 1 Data Analysis:'''
For this assignment, you will use sample data to answer the question: '''Do men and women have different body temperatures?'''
 
The file '''temps.txt''' located in ../bio425_2011/data on eniac, contains body temperature data for a sample of adults.
 
Use a hypotheses test with α = .05 to answer the above question of interest.
 
NOTE: For this part of the assignment you will need to turn in your answer to the question with p-values in addition to the R syntax used. '''Indicate your null hypothesis'''.
 
 
'''Part 2 Gene Expression Data Analysis:'''
 
Using the files '''GSM129276_cy3.txt''' & '''GSM129276_cy5.txt''' located in ./bio425_2011/data on eniac, conduct an analysis to produce a histogram of fold changes.
 
In addition to the histogram, you will need to turn in the R syntax used in every step of the analysis in R, along with an explanation as to why the step was necessary.
 
|-style="background-color:powderblue;"
| '''Read'''
'''For next class, read CH 7'''
|}


===May 14===
===May 14===
*Chapter 7. Protein Structure Prediction
*'''Chapter 7.''' Protein Structure Prediction
<!--*Assignment #14 (Final Comprehensive Project). '''(To be posted)'''-->
<!--*Assignment #14 (Final Comprehensive Project). '''(To be posted)'''-->


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*Final Project Due (TBA)
*Final Project Due (TBA)


==Useful Links==


==Useful Links==
===Unix Tutorials===
*A very nice [http://info.ee.surrey.ac.uk/Teaching/Unix/ UNIX tutorial] (you will only need up to, and including, tutorial 4).
*A very nice [http://www.ee.surrey.ac.uk/Teaching/Unix/ UNIX tutorial] (you will only need up to, and including, tutorial 4).
*FOSSWire's [http://files.fosswire.com/2007/08/fwunixref.pdf Unix/Linux command reference] (PDF). Of use to you: "File commands", "SSH", "Searching" and "Shortcuts".
*FOSSWire's [http://files.fosswire.com/2007/08/fwunixref.pdf Unix/Linux command reference] (PDF). Of use to you: "File commands", "SSH", "Searching" and "Shortcuts".
===Perl Help===
* Professor Stewart Weiss has taught CSCI132, a UNIX and Perl class. His slides go into much greater detail and are an invaluable resource. They can be found on his course page [http://compsci.hunter.cuny.edu/~sweiss/course_materials/csci132/csci132_f10.php here].
* Perl documentation at [http://perldoc.perl.org perldoc.perl.org]. Besides that, running the perldoc command before either a function (with the -f option ie, perldoc -f substr) or a perl module (ie, perldoc Bio::Seq) can get you similar results without having to leave the terminal.
===Bioperl===
* BioPerl's [http://www.bioperl.org/wiki/HOWTOs HOWTOs page].
* BioPerl-live [http://doc.bioperl.org/bioperl-live developer documentation]. (We use bioperl-live in class.)
* Yozen's tutorial on [http://diverge.hunter.cuny.edu/wiki/HOWTO:Bioperl-live_on_Mac_OS_X installing bioperl-live on your own Mac OS X machine]. (Let me know if there are any issues!).
* [https://spreadsheets.google.com/pub?key=0AjfPzjrqY7BndHpyRHlDZUlGcktINm1IbXVzX1QzMXc&single=true&gid=0&output=html A small table] showing some methods for BioPerl modules with usage and return values.
===SQL===
* [https://docs.google.com/document/d/1zYLPeenwsqPYchkpXnndzphBbTKqX2GjjLHDxlBnt78/edit?hl=en&authkey=CLnh_88K SQL Primer], written by Yozen.
===R Project===
* Install location and instructions for [http://lib.stat.cmu.edu/R/CRAN/bin/windows/base/ Windows]
* Install location and instructions for [http://lib.stat.cmu.edu/R/CRAN/ Mac OS X]
* For users of Ubuntu/Debian:
sudo apt-get install r-base-core
* For users of Fedora/Red Hat:
su -
yum install R
===Utilities===
*An [https://chrome.google.com/webstore/detail/nlbjncdgjeocebhnmkbbbdekmmmcbfjd RSS button extension] for chrome. Can add feeds to Google Reader and others.
*A [https://chrome.google.com/webstore/detail/hcamnijgggppihioleoenjmlnakejdph similar extension] which adds a "Live bookmarks"-like feature to Chrome (like Firefox's RSS bookmarks).
===Other Resources===
* [http://www.ccrnp.ncifcrf.gov/~toms/papers/primer/primer.pdf Information Theory Primer] by Thomas D. Schneider. Useful in understanding sequence logo maps.




© Weigang Qiu, Hunter College, Last Update Jan 2011
© Weigang Qiu, Hunter College, Last Update Jan 2011

Latest revision as of 02:05, 10 May 2011

Computational Molecular Biology

BIOL 425/790.49, Spring 2011

Hunter College of the City University of New York

The best way to track changes made to this page is to click on the History tab above, and add the RSS feed that shows up there in your browser. If you use Chrome, please see #Useful Links below for two very handy extensions if you don't have them, or other RSS extensions, already.


Course information

Instructors: Che Martin and Yozen Hernandez

Class Hours: Room 1000G HN; Saturday 11am-2pm

Office Hours: Room 830 HN; Thursdays 12-2pm or by appointment

Contact information:

  • Che: cmartin@gc.cuny.edu, 917-684-0864
  • Yozen: yzhernand@gmail.com, 347-829-6936


Course Description

Background

Biomedical research is becoming a high-throughput science. As a result, information technology plays an increasingly important role in biomedical discovery. Bioinformatics is a new interdisciplinary field formed between molecular biology and computer science.

Contents

This course will introduce both bioinformatics theories and practices. Topics include: database searching, sequence alignment, molecular phylogenetics, structure prediction, and microarray analysis. The course is held in a UNIX-based instructional lab specifically configured for bioinformatics applications. Each session consists of a first-half instruction on bioinformatics theories and a second-half session of hands-on exercises.

Learning Goals

Students are expected to be able to:

  • Approach biological questions evolutionarily ("Tree-thinking")
  • Evaluate and interpret computational results statistically ("Statistical-thinking")
  • Formulate informatics questions quantitatively and precisely ("Abstraction")
  • Design efficient procedures to solve problems ("Algorithm-thinking")
  • Manipulate high-volume textual data using UNIX tools, Perl/BioPerl, R, and Relational Database ("Data Visualization")

Pre-requisites

This 3-credit course is designed for upper-level undergraduates and graduate students. Prior experiences in the UNIX Operating System and at least one programming language are required. Hunter pre-requisites are CSCI132 (Practical Unix and Perl Programming) and BIOL300 (Biochemistry) or BIOL302 (Molecular Genetics), or permission by the instructor.

Textbook

Krane & Raymer (2003). Fundamental Concepts of Bioinformatics. Pearson Education, Inc. (ISBN 0-8053-4633-3)

This book should be available in the Hunter Bookstore, as well as through several popular retailers and resellers online.

Grading & Academic Honesty

Hunter College regards acts of academic dishonesty (e.g., plagiarism, cheating on examinations, obtaining unfair advantage, and falsification of records and official documents) as serious offenses against the values of intellectual honesty. The College is committed to enforcing the CUNY Policy on Academic Integrity and will pursue cases of academic dishonesty according to the Hunter College Academic Integrity Procedures.

Student performance will be evaluated by weekly assignments and projects. While these are take-home projects and students are allowed to work in groups and answers to some of the questions are provided in the back of the textbook, students are expected to compose the final short answers, computer commands, and code independently. There are virtually an unlimited number of ways to solve a computational problem, as are ways and personal styles to implement an algorithm. Writings and blocks of codes that are virtually exact copies between individual students will be investigated as possible cases of plagiarism (e.g., copies from the Internet, text book, or each other). In such a case, the instructor will hold closed-door exams for involved individuals. Zero credits will be given to ALL involved individuals if the instructor considers there is enough evidence for plagiarism. To avoid being investigated for plagiarism, Do Not Copy from Others & Do Not Let Others Copy Your Work.

Submit assignments in Printed Hard Copies. Email attachments will NOT be accepted. Each assignment will be graded based on timeliness (10%), completeness (30%), whether executable or having major errors (20%), correctness of the final output (20%), algorithm efficiency (10%), and cleanness and readability in programming styles (10%).

The grading scheme for the course, is as follows (Subject to some change. You will be notified with sufficient time):

  • Assignments (50%): 10 exercises.
  • Mid-term (20%): In class Assignment + Take home to be collected on the same day.
  • Final exam (20%)
  • Classroom Q & A (5%): Read the chapters before lecture.
  • Attendance (5%): 1-2 absences = -2.5%. More than 2 = -5%.

Programming Assignment Expectations

All code must begin with the lines in the Perl slides, without exception. For each assignment, unless otherwise stated, I would like the full text of the source code. Since you cannot print using the text editor in the lab (even if you are connected from home), you must copy and paste the code into a word processor or a local text editor. If you are using a word processor, change the font to a fixed-width/monospace font. On Windows, this is usually Courier.

Code indentation is your personal taste, so long as it is consistent and readable. Use comments whenever you think either the code is unclear, or simply as a guideline for yourself. Well-commented code improves readability, but be careful not overdo it.

Also, unless otherwise stated, both the input and the output of the program must be submitted as well. This should also be in fixed-width font, and you should label it in such a way so that I know it is the program's input/output. This is so that I know that you've run the program, what data you have used, and what the program produced.

If you are working from the lab, one option is to email the code to yourself, change the font, and then print it somewhere else as there is no printer in the lab.

Course Schedule (All Saturdays)

Dates and assignments below are subject to some change

"Lecture slides" links will be available either during or before each lecture, in PDF.

Homework assignments are due the week *after* the date under which they appear. ie, an assignment posted under Jan 29 is due the following lecture, on Feb 5.

January 29

  • Course Overview
  • Tutorial: UNIX Account, Tools, & Emacs [Lecture Slides]
  • UNIX Tutorial: Please check the new #Useful Links section below
  • How to connect remotely: (Windows) (Mac)
  • Homework: This homework will *not* be graded. It is for practice purposes ONLY.

February 5

February 12

NO CLASS

(Read Chapter 6 for next class)

February 19

Yozen will not be lecturing

  • Chapter 6. Gene and Genome Structures [Lecture Slides Lecture Slides Ch.6-Che
  • Tutorial: ORF Prediction using GLIMMER
  • Homework: This homework will be graded.

February 26

March 5

March 12

March 19

  • REVIEW Session for MID-TERM EXAMS

March 26

  • MID-TERM

April 2

April 9

April 16

  • Topic: Relational Database and SQL
  • Tutorial: the Borrelia Genome Database
  • Homework: SQL-embedded PERL

April 23

NO CLASSES (Spring recess)

April 30

May 7

  • Chapter 6 (Gene Expression) & Chapter 8 (Proteomics)
  • Tutorial: Array Data Visualization and Analysis ( Micro-Array Analysis Slides)
  • Homework:Data Analysis using R

May 14

  • Chapter 7. Protein Structure Prediction

May 21

  • Final Project Due (TBA)

Useful Links

Unix Tutorials

Perl Help

  • Professor Stewart Weiss has taught CSCI132, a UNIX and Perl class. His slides go into much greater detail and are an invaluable resource. They can be found on his course page here.
  • Perl documentation at perldoc.perl.org. Besides that, running the perldoc command before either a function (with the -f option ie, perldoc -f substr) or a perl module (ie, perldoc Bio::Seq) can get you similar results without having to leave the terminal.

Bioperl

SQL

R Project

  • Install location and instructions for Windows
  • Install location and instructions for Mac OS X
  • For users of Ubuntu/Debian:
sudo apt-get install r-base-core
  • For users of Fedora/Red Hat:
su -
yum install R

Utilities

Other Resources


© Weigang Qiu, Hunter College, Last Update Jan 2011

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