Revision as of 15:42, 21 February 2013

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 =BIOL 202=
 =BIOL 300=
-=BIOL 302 (Molecular Genetics)=
-==Identification of mdm2 Splice Variants Using BLAST==
-===Objectives===
-* Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
-* Use BLAST to identify
-===Key Concepts===
-====Blast====
-====Alternative Splicing====
-===Exercise===
-Scenario: You work in a lab, and your research group has isolated four mRNA sequences...
-{| class="wikitable"
-! Genbank Accession # !! cDNA Clone !! Description !! Cell Line !! Length (bp)
-|-
-| AF527840 ||		 || Genomic DNA			 || 	 	|| 34,088
-|-
-| EU076746 || P2-MDM2-C1 || cDNA missing exons 5-9 & 11  || MANCA	|| 427
-|-
-| EU076747 || P2-MDM2-10 || cDNA missing exon 10	 || ML-1	|| 842
-|-
-| EU076748 || P2-MDM2-C  || cDNA missing exons 5-9	 || A876	|| 505
-|-
-| EU076749 || P2-MDM2-FL || Full-length cDNA		 || SJSA-1	|| 845
-|-
-|}
-[[File:4seq.png]]
-===Questions===

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