Pseudomonas population genomics: Difference between revisions
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| fleN || [[fleN pep alignment]] ||[[File:flen.png|200px]] || [http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=live&SEQUENCE=AEV61607.1 Conserved Domain] | | fleN || [[fleN pep alignment]] ||[[File:flen.png|200px]] || [http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=live&SEQUENCE=AEV61607.1 Conserved Domain] | ||
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| fleQ || [[ | | fleQ || [[fleQ pep alignment]] ||[[File:fleq.png|200px]] || [http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=live&SEQUENCE=AEV61607.1 Conserved Domain] | ||
|- | |- | ||
| flhf ||[[ | | flhf ||[[flhF pep alignment]]||[[File:flhf.png|200px]] || [http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=live&SEQUENCE=AEV61607.1 Conserved Domain] | ||
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Revision as of 20:35, 18 June 2013
Projects
- Build a local genome database
- Database schema:
- "genome": genome_id, strain_name, ncbi_taxid
- "orf": genome_id, locus_tag, start, stop, strand, genome_name, product_name
- "orth_orf": orth_orf_id, locus_name, genome_id, orth_class
- Parsing scripts
- Rayees Parsing code, requires that you remove columns 9-27 using bash command:
cut -c 1-8
(I will write a bash script that does this and runs the program) https://www.dropbox.com/s/lpxxbkxeyw7frrn/parser.pl
- Rayees Parsing code, requires that you remove columns 9-27 using bash command:
- Database loading scripts
- Database schema:
- Molecular Evolution of flagellum genes
- Download orthologs
- Reconstruct phylogenetic tree
- Run PAML tests
Update: June 18, 2013
- Material and Methods
- Web links & how did you download the sequences?
- Protocol: Sequence name editing/rule (script name?); Alignment (tool and command); Phylogeny; Tree display (R commands)
Gene | Alignment | Tree | Notes |
---|---|---|---|
fleN | fleN pep alignment | Conserved Domain | |
fleQ | fleQ pep alignment | Conserved Domain | |
flhf | flhF pep alignment | Conserved Domain |
Benchmark: June 11, 2013
- Finish parsing the genome files to upload the "orf" table (Raymond & Rayees)
- Rayees Parsed genome files: https://www.dropbox.com/sh/k0zktvvmv39op9i/1zBercEky8
- Parsing the ortholog file to upload the "orth_orf" table (Raymond)
- Identify and download fleN, fleQ, and flhF orthologs & align them (Rayees)