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imported>Ppagan (Created page with "=BIOL 100= =BIOL 102= =BIOL 200= =BIOL 202= =BIOL 300= =BIOL 302 (Molecular Genetics)= ==Identification of mdm2 Splice Variants Using BLAST== ===Learning Objectives=== ===...") |
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==Identification of mdm2 Splice Variants Using BLAST== | ==Identification of mdm2 Splice Variants Using BLAST== | ||
===Objectives=== | |||
* Learn to use Genbank database and BLAST tool to analyze nucleotide sequences | |||
* Use BLAST to identify | |||
===Key Concepts=== | |||
====Blast==== | |||
====Alternative Splicing==== | |||
=== | ===Exercise=== | ||
Scenario: You work in a lab, and your research group has isolated four mRNA sequences... | |||
{| class="wikitable" | {| class="wikitable" | ||
! Genbank Accession # !! cDNA Clone !! Description !! Cell Line !! Length (bp) | ! Genbank Accession # !! cDNA Clone !! Description !! Cell Line !! Length (bp) | ||
| Line 30: | Line 35: | ||
[[File:4seq.png]] | [[File:4seq.png]] | ||
===Questions=== | |||
Revision as of 19:24, 7 February 2013
BIOL 100
BIOL 102
BIOL 200
BIOL 202
BIOL 300
BIOL 302 (Molecular Genetics)
Identification of mdm2 Splice Variants Using BLAST
Objectives
- Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
- Use BLAST to identify
Key Concepts
Blast
Alternative Splicing
Exercise
Scenario: You work in a lab, and your research group has isolated four mRNA sequences...
| Genbank Accession # | cDNA Clone | Description | Cell Line | Length (bp) |
|---|---|---|---|---|
| AF527840 | Genomic DNA | 34,088 | ||
| EU076746 | P2-MDM2-C1 | cDNA missing exons 5-9 & 11 | MANCA | 427 |
| EU076747 | P2-MDM2-10 | cDNA missing exon 10 | ML-1 | 842 |
| EU076748 | P2-MDM2-C | cDNA missing exons 5-9 | A876 | 505 |
| EU076749 | P2-MDM2-FL | Full-length cDNA | SJSA-1 | 845 |
