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==Identification of mdm2 Splice Variants Using BLAST==
==Identification of mdm2 Splice Variants Using BLAST==
===Objectives===
===[[QuBi/modules/biol302#Objectives|Objectives]]===
* Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
* Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
* Use BLAST to identify  
* Use BLAST to identify  


===Key Concepts===
===[[QuBi/modules/biol302#Key Concepts|Key Concepts]]===
====Blast====
====Blast====
====Alternative Splicing====
====Alternative Splicing====

Revision as of 16:27, 21 February 2013

BIOL 302 Lab: Bioinformatics Exercises

[Link to BIOL302 page, if one exists]


Identification of mdm2 Splice Variants Using BLAST

Objectives

  • Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
  • Use BLAST to identify

Key Concepts

Blast

Alternative Splicing

Exercise

NOTE: Maybe we should remove the "Description" section of this table and leave it up to the students to figure out which exons are missing

Genbank Accession # cDNA Clone Description Cell Line Length (bp)
AF527840 Genomic DNA 34,088
EU076746 P2-MDM2-C1 cDNA missing exons 5-9 & 11 MANCA 427
EU076747 P2-MDM2-10 cDNA missing exon 10 ML-1 842
EU076748 P2-MDM2-C cDNA missing exons 5-9 A876 505
EU076749 P2-MDM2-FL Full-length cDNA SJSA-1 845
Explore the annotation for AF527840.

The sequences on the genbank database have annotations associated with them. Some sequences are richly annotated to contain all of the details you would want, and other sequences may have almost no annotations at all. The sequence you are about to explore is well annotated with details such as exon locations and nucleotide variablility. Use the annotations to complete the following tasks and questions.

1) DRAW a diagram of this gene, including its introns/exons, 3'/5' UTRs, +1. (Note: this diagram is going to be very handy for the last set of questions.) - Label each feature with its coordinates. For example, if an exon starts at 500bp and ends at 1000bp, label it as such. - Label the diagram with basic information, such as the gene's name and th organism's species. - The drawing does not have to be exactly to scale, a reasonable effort should be made to do so. Put length markers on your drawing (for example, every 5000bp.) - The mRNA annotation states which segments are used to create mRNA, and the CDS annotation states which parts actully code amino acids (CDS = coding sequence).

2) How does the sequence vary at positions X, X, and X for this gene?

3) What kinds of repeat regions can be found in this gene?

4seq.png

Questions