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BIOL 100
BIOL 102
BIOL 200
BIOL 202
BIOL 300
BIOL 302 (Molecular Genetics)
Identification of mdm2 Splice Variants Using BLAST
Objectives
- Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
- Use BLAST to identify
Key Concepts
Blast
Alternative Splicing
Exercise
Scenario: You work in a lab, and your research group has isolated four mRNA sequences...
| Genbank Accession # | cDNA Clone | Description | Cell Line | Length (bp) |
|---|---|---|---|---|
| AF527840 | Genomic DNA | 34,088 | ||
| EU076746 | P2-MDM2-C1 | cDNA missing exons 5-9 & 11 | MANCA | 427 |
| EU076747 | P2-MDM2-10 | cDNA missing exon 10 | ML-1 | 842 |
| EU076748 | P2-MDM2-C | cDNA missing exons 5-9 | A876 | 505 |
| EU076749 | P2-MDM2-FL | Full-length cDNA | SJSA-1 | 845 |
