Annotate-a-genome
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Project Goals
- Annotate and add newly sequenced Borrelia genomes to BorreliaBase
- Build an informatics pipeline for gene prediction, ortholog calls, databasing, and synteny analysis
Download genome sequences from GenBank
Strain | Species | Group | Genome Sequences | Notes |
---|---|---|---|---|
B31 | B. burgdorferi (reference genome) | Lyme Disease | Example | |
CA382 | B. burgdorferi (California) | Lyme Disease | Example | |
CA8 | B. burgdorferi (California) | Lyme Disease | Example | |
BgVir | B. garinii (Russia) | Lyme Disease | Example | |
NMJW1 | B. garinii (China) | Lyme Disease | Example | |
HLJ01 | B. afzelii (China) | Lyme Disease | Example | |
Ly | B. duttonii (Tanzania) | Relapsing Fever |
|
Example |
A1 | B. recurrentis (Ethiopia) | Relapsing Fever | Example | |
DAH | B. hermsii (Washington State) | Relapsing Fever | Example | |
91E135 | B. turicatae (Texas) | Relapsing Fever | Example | |
Achema | B. crocidurae (Mauritania) | Relapsing Fever |
|
Example |
HR1 | B. parkeri (??) | Relapsing Fever | Example | |
LB-2001 | B. miyamotoi (Northeast US) | Relapsing Fever | Example |
Protocol
- Predict genes with Glimmer if necessary
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$v = "string"; // sample initialization
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echo $v; // end of php code
?>
- Predict orthologs with reciprocal BLAST
- Load into database
- Visualize results using synteny browser