NYRaMP-Informatics-2025

NYRaMP Informatics Workshop August 2025, Tuesdays 9:30-11:30, DNA Learning Center Instructors: Brandon Ely (CUNY Graduate Center, bely@gradcenter.cuny.edu)

MA plot	Volcano plot	Heat map
fold change (y-axis) vs. total expression levels (x-axis)	p-value (y-axis) vs. fold change (x-axis)	genes significantly down or up-regulated (at p<1e-4)

Overview

A genome is the total genetic content of an organism. Driven by breakthroughs such as the decoding of the first human genome and rapid DNA and RNA-sequencing technologies, biomedical sciences are undergoing a rapid & irreversible transformation into a highly data-intensive field, that requires familiarity with concepts in both biological, computational, and statistical sciences.

Genome information is revolutionizing virtually all aspects of life Sciences including basic research, medicine, and agriculture. Meanwhile, use of genomic data requires life scientists to be familiar with concepts and skills in biology, computer science, as well as statistics.

This workshop is designed to introduce computational analysis of genomic data through hands-on computational exercises.

Learning goals

By the end of this workshop students will be able to:

• Manipulate data with R & Rstudio
• Visualize data using R & RStudio
• Analyze microbiome data

Web Links

• Cloud R account (free): https://posit.cloud/; Join the shared work space "NYRaMP-Informatics"
• For your own computer, download the desktop version: https://posit.co/download/rstudio-desktop/
• Textbook: Introduction to R for Biologists
• Download: R datasets
• A reference book: R for Data Science (Wickharm & Grolemund)

Week 1. Aug 5

• Pre-test: visualization, interpretation, and stats. Download file: File:Pre-test.pdf
• Computer/Cloud setup & software download/installation
• R Tutorial 1. Getting started: Basics: interface, packages, variables, objects, functions. Download slides: File:R-tutorials-Part-1.pdf
• Practice #1

Week 2. Aug 12

• R Tutorial 2. Data manipulation. Download slides: File:R-tutorials-Part-2.pdf
• Practice #2

Week 3. Aug 19

• R Tutorial 3. Data visualization. Lecture slides: File:R-tutorials-Part-3.pdf
• Practice #3
• Demo I. Microbiome data analysis