QuBi/modules/bio302: Difference between revisions

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=BIOL 302 (Molecular Genetics)=


==Identification of mdm2 Splice Variants Using BLAST==
===Objectives===
* Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
* Use BLAST to identify
===Key Concepts===
====Blast====
====Alternative Splicing====
===Exercise===
Scenario: You work in a lab, and your research group has isolated four mRNA sequences...
{| class="wikitable"
! Genbank Accession # !! cDNA Clone !! Description !! Cell Line !! Length (bp)
|-
| AF527840 || || Genomic DNA || || 34,088
|-
| EU076746 || P2-MDM2-C1 || cDNA missing exons 5-9 & 11  || MANCA || 427
|-
| EU076747 || P2-MDM2-10 || cDNA missing exon 10 || ML-1 || 842
|-
| EU076748 || P2-MDM2-C  || cDNA missing exons 5-9 || A876 || 505
|-
| EU076749 || P2-MDM2-FL || Full-length cDNA || SJSA-1 || 845
|-
|}
[[File:4seq.png]]
===Questions===

Latest revision as of 15:54, 21 February 2013