QuBi/modules/bio302

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BIOL 302 (Molecular Genetics)

Identification of mdm2 Splice Variants Using BLAST

Objectives

  • Learn to use Genbank database and BLAST tool to analyze nucleotide sequences
  • Use BLAST to identify

Key Concepts

Blast

Alternative Splicing

Exercise

Scenario: You work in a lab, and your research group has isolated four mRNA sequences...

Genbank Accession # cDNA Clone Description Cell Line Length (bp)
AF527840 Genomic DNA 34,088
EU076746 P2-MDM2-C1 cDNA missing exons 5-9 & 11 MANCA 427
EU076747 P2-MDM2-10 cDNA missing exon 10 ML-1 842
EU076748 P2-MDM2-C cDNA missing exons 5-9 A876 505
EU076749 P2-MDM2-FL Full-length cDNA SJSA-1 845

4seq.png

Questions