Year 2019: Difference between revisions
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imported>Lab |
imported>Weigang m (→TD Project) |
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| Line 1: | Line 1: | ||
=TD Project= | =TD Project= | ||
* Credit: Christopher Panlasigui | * Credit: Christopher Panlasigui | ||
* | * Brief computational/statistical steps: | ||
# Quantile normalization between all replicates (with log2 transformatuon) | # Quantile normalization between all replicates (with log2 transformatuon) | ||
# Linear model among the 3 groups | # Linear model among the 3 groups | ||
| Line 7: | Line 7: | ||
# Map gene names | # Map gene names | ||
# Cluster and show by interactive heatmaps in R | # Cluster and show by interactive heatmaps in R | ||
* Results | |||
## [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html Heatmap 1. Scaled per row] | ## [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html Heatmap 1. Scaled per row] | ||
## [http://diverge.hunter.cuny.edu/~weigang/heatmap_fc_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-3). Values are Fold Change over mean Wild Tyoe] | ## [http://diverge.hunter.cuny.edu/~weigang/heatmap_fc_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-3). Values are Fold Change over mean Wild Tyoe] | ||
Revision as of 16:25, 8 February 2019
TD Project
- Credit: Christopher Panlasigui
- Brief computational/statistical steps:
- Quantile normalization between all replicates (with log2 transformatuon)
- Linear model among the 3 groups
- Select top significant genes
- Map gene names
- Cluster and show by interactive heatmaps in R
- Results