Year 2019: Difference between revisions

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imported>Lab
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* Results
* Results
# [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html Heatmap 1. Scaled by gene]
# [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html Heatmap 1. Scaled by gene]
# [http://diverge.hunter.cuny.edu/~weigang/heatmap_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-3). Values are Fold Change over mean Wild Type]
# [http://diverge.hunter.cuny.edu/~weigang/new_heatmap_td.html heatmap of top differentiately expressed genes (p<0.001, 1e-5). Values are Fold Change over mean Wild Type]

Revision as of 17:11, 8 February 2019

TD Project

  • Credit: Christopher Panlasigui
  • Brief computational/statistical steps:
  1. Quantile normalization between all replicates (with log2 transformatuon)
  2. Linear model among the 3 groups
  3. Select top significant genes
  4. Map gene names
  5. Cluster and show by interactive heatmaps in R
  • Results
  1. Heatmap 1. Scaled by gene
  2. heatmap of top differentiately expressed genes (p<0.001, 1e-5). Values are Fold Change over mean Wild Type