Undergrad Research Experience: Difference between revisions

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imported>Weigang
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** [https://msphere.asm.org/content/3/2/e00089-18 Fletcher et al (2018).]
** [https://msphere.asm.org/content/3/2/e00089-18 Fletcher et al (2018).]
** [https://www.sciencedirect.com/science/article/pii/S0944501318301988?via%3Dihub Gu et al (2018)]
** [https://www.sciencedirect.com/science/article/pii/S0944501318301988?via%3Dihub Gu et al (2018)]
* Data set: on "genometracker.org"
* Data set: posted on "genometracker.org"
** Wild type transcriptome at 12 hour, paired-end read files:
** /home/azureuser/18134XR-29-01_S0_L001_R1_001.fastq.gz
** /home/azureuser/18134XR-29-01_S0_L001_R2_001.fastq.gz
* To Do
* To Do
** 9/8/2020 12 noon: Demo on read quality using FastQC and mapping reads to reference genomes with bowtie
** 9/8/2020 12 noon: Demo on read quality using FastQC and mapping reads to reference genomes with bowtie

Revision as of 21:01, 1 September 2020

Fall 2020

Participants

  • 'Eamen Ho': Volunteer research assistant
  • 'Ramandeep Singh': BIOL 48002
  • 'Desiree Pante': BIOL 48001
  • 'Afsana Rahman': Volunteer research assistant
  • 'Roman Shimonov': BIOL 48002
  • 'Justin Hiraldo': BIOL 48002
  • 'Zaheen Hossain': Volunteer research assistant

Schedule

  • Tuesdays at 12 noon - 2pm by Zoom
  • Sept 1, 2020. Week 1. Meet & Greet; Intro to projects
  • Sept 8, 2020. Week 2. Presentations (background, data, and methods), based on assigned readings

Project 1. Structure & evolution of multipartite genome of Lyme disease bacteria

  • Participants: Desiree & Ramon
  • Readings
  • Data set: lp54 & cp26 plasmids
  • TO DO:
    • 9/8/2020, 12 noon: 5-slides presentation on multipartite bacterial genome evolution (based on the paper above)

Project 2. OspC Cross-reactivity analysis

  • Participants: Justin, Roman
  • Readings: Ivanova et al (2009)
  • Tool: ImageJ
  • Data set (to be sent)
  • To Do
    • 9/8/2020 12 noon: 5-slide presentation on background, material & methods, and data capture using ImageJ

Project 3. Clostridium transcriptome analysis

  • Participants: Eaman, Zaheen
  • Readings
  • Data set: posted on "genometracker.org"
    • Wild type transcriptome at 12 hour, paired-end read files:
    • /home/azureuser/18134XR-29-01_S0_L001_R1_001.fastq.gz
    • /home/azureuser/18134XR-29-01_S0_L001_R2_001.fastq.gz
  • To Do
    • 9/8/2020 12 noon: Demo on read quality using FastQC and mapping reads to reference genomes with bowtie