Strain natural frequency: Difference between revisions
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imported>Weigang (Created page with "==Current status== * Two tick populations from NY, Fall 2011 & Fall 2012 * Genomic DNA controls: A & K * Tested for Bb presence by PCR * 454 sequencing data generated ==Plans...") |
imported>Weigang m (→Plans) |
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==Plans== | ==Plans== | ||
* Manuscript drafting (Summer 2013) | * Manuscript drafting (Summer 2013) | ||
** Background | |||
*** Traditional method: Reverse-Line Blotting (tedious, see [http://www.ncbi.nlm.nih.gov/pubmed/11901105 Qiu et al 1999] and [http://www.ncbi.nlm.nih.gov/pubmed/15514047 Brisson & Dykhuizen 2004]) | |||
** Material & Methods | ** Material & Methods | ||
*** Tick collection and field work | *** Tick collection and field work | ||
Latest revision as of 19:37, 24 June 2013
Current status
- Two tick populations from NY, Fall 2011 & Fall 2012
- Genomic DNA controls: A & K
- Tested for Bb presence by PCR
- 454 sequencing data generated
Plans
- Manuscript drafting (Summer 2013)
- Background
- Traditional method: Reverse-Line Blotting (tedious, see Qiu et al 1999 and Brisson & Dykhuizen 2004)
- Material & Methods
- Tick collection and field work
- Tick DNA
- Primer design/PCR
- 454 sequencing
- Allele identification
- Statistical analysis: correlation analysis & Fst tests
- Results
- Background
- Wet-lab work (Summer 13)
- DNA prep for the Fall 2012 population, N=50 ticks
- Try paired-ends illumina and compare with 454: A reference
- Fieldwork: Fall 2013