Strain natural frequency

Current status

• Two tick populations from NY, Fall 2011 & Fall 2012
• Genomic DNA controls: A & K
• Tested for Bb presence by PCR
• 454 sequencing data generated

Plans

• Manuscript drafting (Summer 2013)
  • Background
    • Traditional method: Reverse-Line Blotting (tedious, see Qiu et al 1999 and Brisson & Dykhuizen 2004)
  • Material & Methods
    • Tick collection and field work
    • Tick DNA
    • Primer design/PCR
    • 454 sequencing
    • Allele identification
    • Statistical analysis: correlation analysis & Fst tests
  • Results
• Wet-lab work (Summer 13)
  • DNA prep for the Fall 2012 population, N=50 ticks
  • Try paired-ends illumina and compare with 454: A reference
• Fieldwork: Fall 2013