Strain natural frequency

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Revision as of 16:52, 31 May 2013 by imported>Weigang (Created page with "==Current status== * Two tick populations from NY, Fall 2011 & Fall 2012 * Genomic DNA controls: A & K * Tested for Bb presence by PCR * 454 sequencing data generated ==Plans...")
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Current status

  • Two tick populations from NY, Fall 2011 & Fall 2012
  • Genomic DNA controls: A & K
  • Tested for Bb presence by PCR
  • 454 sequencing data generated

Plans

  • Manuscript drafting (Summer 2013)
    • Material & Methods
      • Tick collection and field work
      • Tick DNA
      • Primer design/PCR
      • 454 sequencing
      • Allele identification
      • Statistical analysis: correlation analysis & Fst tests
    • Results
  • Wet-lab work (Summer 13)
    • DNA prep for the Fall 2012 population, N=50 ticks
    • Try paired-ends illumina and compare with 454: A reference
  • Fieldwork: Fall 2013